nasiona marihuany

The inheritance of chemical phenotype in Cannabissativa L. (IV): cannabinoid-free pla

Wyszukiwarka Forumowa:
S

sub

Guest
The inheritance of chemical phenotype in Cannabissativa L. (IV): cannabinoid-free plants

E. P. M. de Meijer1 , K. M. Hammond1 and A. Sutton1

(1) GW Pharmaceuticals Plc., Porton Down Science Park, Salisbury, Wiltshire, SP4 0JQ, UK

Received: 28 September 2008 Accepted: 15 January 2009 Published online: 31 January 2009


Abstract:

A genetic factor that blocks the cannabinoid biosynthesis in Cannabis sativa has been investigated. Crosses between cannabinoid-free material and high content, pharmaceutical clones were performed. F1s were uniform and had cannabinoid contents much lower than the mean parental value. Inbred F2 progenies segregated into discrete groups: a cannabinoid-free chemotype, a chemotype with relatively low cannabinoid content and one with relatively high content, in a monogenic 1:2:1 ratio. In our model the cannabinoid knockout factor is indicated as a recessive allele o, situated at locus O, which segregates independently from previously presented chemotype loci. The genotype o/o underlies the cannabinoid-free chemotype, O/o is expressed as an intermediate, low content chemotype, and O/O is the genotype of the high content chemotype. The data suggests that locus O governs a reaction in the pathway towards the phenolic cannabinoid precursors. The composition of terpenoids and various other compound classes of cannabinoid-free segregants remains unaffected. Backcrossing produced cannabinoid-free homologues of pharmaceutical production clones with potential applications in pharmacological research. A new variant of the previously presented allele ‘B 0’, that almost completely obstructs the conversion of CBG into CBD, was also selected from the source population of the cannabinoid knockout factor.

Keywords Cannabinoids - Cannabis - Chemotype - Genotype - Knockouts
 



Z kodem HASZYSZ dostajesz 20% zniżki w sklepie Growbox.pl na wszystko!

nasiona marihuany
Góra Dół